Quantitative Synthetic Ureaplasma urealyticum DNA can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load. Preparation includes fragments from 16S rRNA, urease complex gene 1 (ureA), intergenic region 1, urease complex gene 2 (ureB), intergenic region 2, urease complex gene 3 (ureC), urease complex gene 4 (ureG), and multiple banded antigen gene (MBA) regions.